TRUST THE SPECIALISTS

cell line preservation

The first step towards a future together

Our mission is to make our clients happy. We offer our specialised services in genetic preservation and the cloning of dogs and cats, camels and horses, among others. Our team of specialists and veterinarians is made up of animal lovers who fully understand and share the bonds that people develop with their pets.

Pet cloning makes it possible to obtain a genetically healthy animal identical to an existing one. It is a simple and safe process that you can carry out thanks to our cell preservation bank, where you can store biological samples until you decide to use this material for the cloning process.

If you have a best and loyal friend and are interested in animal cloning, our team of professionals can begin the replication process once the DNA sample is received (usually skin tissue). Our team will carry out the cloning in a specialised and secure laboratory using the best protocols and techniques to complete the procedure successfully. In addition, all animals will undergo veterinary tests to ensure they are in good health.

The preservation of pet cell lines makes it possible to obtain a genetically healthy animal identical to an existing one

How is the process of
the preservation of cell lines carried out?

The cloning process is very simple and begins with a DNA sample. It can be a cryopreserved sample or, in the case of a valuable animal with a premature end, from a biopsy (skin tissue) taken immediately after its death. To carry out the process, we need to extract the nucleus from an egg (of the indicated species) in order to fertilise this egg —without its own genes— with the DNA sample. The fusion of this fertilised egg will give rise to an embryo.

CELL LINE PRESERVATION KIT

At Ovoclone, we provide you with a specialised kit for the preservation of cell lines, designed to ensure the highest quality and safety in every process.

We have adapted and fully equipped facilities to carry out any animal cloning or reproduction procedure, following strict biosecurity protocols that ensure the success of each stage.

If you wish, you can request our kit, but it is important to note that the entire procedure must be carried out exclusively by a veterinarian. For this reason, the kit will be sent directly to your trusted veterinarian, who will be responsible for collecting and handling the samples in accordance with our recommendations.

1

Acquisition

2www.ovoclone.com

Kit request

3

Kit received

4

Checking the kit contents

5

Store in the freezer
the cold packs
for future shipment

6

Fill in
documentation

7

Biopsy collection

8

Sample shipment

9

Processing
of the sample

10

Delivery of results

FREQUENTLY ASKED QUESTIONS

How long does the cloning process take?

The cloning process itself takes one day. Therefore, to carry out a proper cloning process, we must have fibroblasts, oocytes, and the necessary equipment available to perform it.

Before cloning, the cells can be preserved for an unlimited time, and the storage period can range from years to decades. For example, in our laboratories we have stored samples from specimens that were cryopreserved twenty to thirty years ago.

It will essentially depend on the species to be cloned. In the case of pets such as dogs and cats, the approximate time is ten months, compared to larger animals such as equines. This varies to an approximate time of a year and a half, since it will depend on the gestation period of the animal we want to clone.

To achieve a successful planning process, we need to have viable cells available. Materials such as nails, hair, and teeth do not provide these cells. In our laboratory, we use skin samples to obtain fibroblasts, since it is a less invasive technique and more respectful of animal welfare.

The embryos obtained after a cloning process are transferred to recipient mothers selected by our team. They will undergo continuous pregnancy and health monitoring to ensure the process goes as smoothly as possible. For this reason, they cannot be housed in other facilities.
These recipient mothers, which will be of a size similar to the animal to be cloned, will stay in a place where calm and tranquility are provided throughout the entire gestation process.

Yes, it is possible to clone geldings (gelded horses) or mares with reproductive problems, because to create a clone we only need a single somatic cell—that is, a normal body cell containing all the genetic material needed to produce a new animal.
The main difference between traditional assisted reproduction and cloning is that the former is sexual reproduction. That is, we need an egg cell and a sperm cell to generate an embryo. However, in cloning, which is asexual reproduction, we need an enucleated egg cell—without genetic information—and a somatic cell, which does contain the genetic information needed to generate an embryo. In this way, a new organism is produced with the same genetic information as the original animal.

If your animal is ill, don’t hesitate to collect the cells as soon as possible: that is the most important thing. We can obtain samples from areas not affected by the illness to generate a completely healthy clone.

If your animal has just passed away, the most important thing is not to freeze the body and to contact our team as soon as possible. You still have the possibility to preserve its genetics and opt for cloning. If you cannot count on your veterinarian at such a crucial moment, don’t worry. We make our entire team available to help you. We can send a specialised person to your home to collect the samples, or we can also refer you to a trusted professional nearby who works with us.

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